the red gene and chitinase gene from clone-5 were ligated together with puc118 ( 2fcwwh i / bap ), and then transformed into ecoli . jm109 . the recombinant cells exhibited chitinase activity and shew red fluorescence under uv 5的几丁质酶基因与red唱ene连接后再插入质粒puc18中,转入大肠杆菌m109菌株,得到含有几丁质酶基因和红色基因的重组菌株。
in the pqe60-hyuh, the hyuh is singly controlled by the t5 promotor, while in pt221-hyuh the hyuh is co-expressed with a molecular chaperon . the expression products of recombinant plasmid were further analysis in solubility and activity . a protein band about 50kd was detected by sds-page in the recombinant cell lysate 将pcr扩增得到的hyuhdna片段分别置于载体质粒pqe60的t5启动子和pt221的t7启动子下游,构建了单独表达质粒pqe60-hyuh和融合表达质粒pt221-hyuh,并对两种重组质粒的表达产物进行了可溶性分析和活性测定。